1. Tigriopus heat tolerance

Background:
Both genes and the environment contribute to most phenotypes. This complicates studies of local adaptation: local adaptation occurs when genotypes found in the "home" environment tend to outperform genotypes from an "away" environment. However, because the environment also influences most phenotypes, any phenotypic differences observed among populations cannot be attributed to genetic differences, without first controlling for environmental differences. In this experiment, you will test for differences among tide pools in the heat tolerance of the copepod Tigriopus californicus, both before and after controlling for environmental effects on this trait.

PART 1: Initial thermal tolerance measurements

Day 1

In the field:
Collect Tigriopus from the two shallowest/sunniest and two deepest/ most shaded pools where you can find them in the splash zone of the intertidal. For each pool collect 3 half-full 1 L containers, each containing at least ~100 individuals. Place a datalogger at the bottom of each pool, set to record hourly temperature measurements.

In the lab:
1) Measure heat tolerance by placing indivudual female Tigriopus in pcr tubes half filled with filtered seawater. Test nine females per pool per temperature (1 hour exposure) at a range of temperatures extending from the 'all alive' to 'all dead' temperature, at 0.2˚C intervals. I suggest 34.8, 35.0, 35.2, 35.4 and 35.6˚C. But you should test the upper and lower temperatures first to see if you need to extend the range. Allow 24 hours recovery before assessing survival. Record number alive and dead in this spreadsheet, then use the R-scripts found in Data Analysis and Tools, to test for differences in heat tolerance among pools and to estimate LT50 temperatures.

2) Maintain each container of copepods at 19˚C for 3 weeks, feeding twice per week (Dr K will show you how much)

PART 2: Thermal tolerance after heat shock vs. common garden acclimation

Comments

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For this project, you will end up having 2-3 Pisaster ochraceus in your care; you must also keep your eyes on t...Falsejohnwares2016-06-30T15:22:33+00:00johnwaresjohn wares2016-06-29 15:51:46+00:002016-06-29 15:51:46updated15johnwaresjohn wares2016-06-29 04:26:27+00:002016-06-29 04:26:27updated14johnwaresjohn wares2016-06-28 04:28:38+00:002016-06-28 04:28:38updated13Added tag - hotjohnwaresjohn wares2016-06-24 15:41:01+00:002016-06-24 15:41:01addTag12johnwaresjohn wares2016-06-19 16:30:30+00:002016-06-19 16:30:30updated11johnwaresjohn wares2016-06-14 13:46:34+00:002016-06-14 13:46:34updated10johnwaresjohn wares2016-06-14 13:43:30+00:002016-06-14 13:43:30updated9Added tag - clinejohnwaresjohn wares2016-06-14 13:35:17+00:002016-06-14 13:35:17addTag8Added tag - phylodiversityjohnwaresjohn wares2016-06-14 13:35:16+00:002016-06-14 13:35:16addTag7Added tag - pcrjohnwaresjohn wares2016-06-14 13:35:08+00:002016-06-14 13:35:08addTag6Added tag - barnaclesjohnwaresjohn wares2016-06-14 13:35:07+00:002016-06-14 13:35:07addTag5johnwaresjohn wares2016-06-14 13:34:06+00:002016-06-14 13:34:06updated4johnwaresjohn wares2016-06-14 13:32:58+00:002016-06-14 13:32:58updated3johnwaresjohn wares2016-06-14 13:31:23+00:002016-06-14 13:31:23updated2First createdjohnwaresjohn wares2016-06-14 13:18:57+00:002016-06-14 13:18:57created1wiki2016-06-29T15:51:46+00:00groups/eccofhl/wiki/6f73aFalseBalanus diversity cline/groups/eccofhl/wiki/6f73a/Balanus_diversity_cline.htmljohn wares15 updatesBalanus diversity cline Background A few studies published DNA sequence data from barnacles (B. glandula) collected 15-20 years ago; the best example is here: ...Falsejohnwares2016-06-29T15:51:46+00:00Added tag - limpetsjohnwaresjohn wares2016-06-24 15:41:25+00:002016-06-24 15:41:25addTag10Added tag - diversityjohnwaresjohn wares2016-06-24 15:41:23+00:002016-06-24 15:41:23addTag9Added tag - distributionjohnwaresjohn wares2016-06-24 15:41:21+00:002016-06-24 15:41:21addTag8Added tag - ecologyjohnwaresjohn wares2016-06-24 15:41:20+00:002016-06-24 15:41:20addTag7Added tag - nichejohnwaresjohn wares2016-06-24 15:41:18+00:002016-06-24 15:41:18addTag6Added tag - hotjohnwaresjohn wares2016-06-24 15:41:17+00:002016-06-24 15:41:17addTag5johnwaresjohn wares2016-06-21 16:40:11+00:002016-06-21 16:40:11updated4morgankellyMorgan Kelly2016-06-20 14:33:01+00:002016-06-20 14:33:01updated3morgankellyMorgan Kelly2016-06-20 14:28:46+00:002016-06-20 14:28:46updated2First createdmorgankellyMorgan Kelly2016-06-20 14:11:36+00:002016-06-20 14:11:36created1wiki2016-06-21T16:40:11+00:00groups/eccofhl/wiki/d254bFalseTransect study/groups/eccofhl/wiki/d254b/Transect_study.htmljohn wares10 updatesTransect study In this exercise, you will use transect surveys and environmental data to create climate envelope models for a group of species of your choosing (it ...Falsejohnwares2016-06-21T16:40:11+00:00Added tag - hotmorgankellyMorgan Kelly2016-06-24 15:41:56+00:002016-06-24 15:41:56addTag5Added tag - adaptationmorgankellyMorgan Kelly2016-06-24 15:39:33+00:002016-06-24 15:39:33addTag4morgankellyMorgan Kelly2016-06-14 13:41:13+00:002016-06-14 13:41:13updated3morgankellyMorgan Kelly2016-06-14 01:37:59+00:002016-06-14 01:37:59updated2First createdmorgankellyMorgan Kelly2016-06-14 01:37:42+00:002016-06-14 01:37:42created1wiki2016-06-14T13:41:13+00:00groups/eccofhl/wiki/60339False1. Tigriopus heat tolerance/groups/eccofhl/wiki/60339/1_Tigriopus_heat_tolerance.htmlMorgan Kelly5 updates1. Tigriopus heat tolerance Background: Both genes and the environment contribute to most phenotypes. This complicates studies of local adaptation: local adaptation occurs when ...Falsemorgankelly2016-06-14T13:41:13+00:00hot/groups/eccofhl/search/index.rss?sort=modifiedDate&kind=all&sortDirection=reverse&excludePages=wiki/welcomelist/groups/eccofhl/search/?sort=modifiedDate&kind=all&sortDirection=reverse&excludePages=wiki/welcomeRecent ChangesRecentChangesListUpdates?sort=modifiedDate&kind=all&sortDirection=reverse&excludePages=wiki/welcome0/groups/eccofhl/sidebar/RecentChangesListmodifiedDateallRecent ChangesRecentChangesListUpdateswiki/welcomeNo recent changes.reverse5searchlist/groups/eccofhl/calendar/Upcoming EventsUpcomingEventsListEvents1Getting events…